FIGURE

Fig. 5

ID

ZDB-FIG-220829-48

Publication

Sheng et al., 2022 - Sema4C Is Required for Vascular and Primary Motor Neuronal Patterning in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Knockout of sema4C caused both aberrant phenotypes in PMNs and ISVs. (A). The targeting site of the gRNA on the sema4C gene; (B). The measure of cutting efficiency using genomic DNAs of injected embryonic (n = 10); (C). Confocal imaging analysis of PMNs and ISVs in control and the F0 generation of the injected Tg(mnx1:EGFP::kdrl:ras-mCherry) zebrafish at 48 hpf, blue arrowheads indicate aberrant PMN and ISV; (D). The statistical analysis of the proportion of abnormal axonal trajectories of Caps (*** p = 0.0006) and short Caps (*** p = 0.0006) in the different groups at 48 hpf. Mann–Whitney test; (E). The statistical analysis of the proportion of vessels that extend across different segments (*** p = 0.0006) and the proportion of proliferated vessels (*** p = 0.006) in the different groups at 48 hpf. Mann–Whitney test.

Expression Data

Genes:	EGFP mCherry
Fish:	ml2Tg/ml2Tg; s916Tg/s916Tg + CRISPR1-sema4c
Knockdown Reagent:	CRISPR1-sema4c
Anatomical Terms:	CaP motoneuron axon intersegmental vessel primary motor neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ml2Tg/ml2Tg; s916Tg/s916Tg + CRISPR1-sema4c
Knockdown Reagent:	CRISPR1-sema4c
Observed In:	CaP motoneuron axon intersegmental vessel primary motor neuron
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cells