FIGURE

Fig. 5

ID

ZDB-FIG-220725-30

Publication

Osorio-Méndez et al., 2022 - Voltage-gated sodium channel scn8a is required for innervation and regeneration of amputated adult zebrafish fins

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Fig. 5

Early fin regeneration is impaired in temca mutants. (A) Schematic of temperature shift assay. (B) Representative whole-mount images of control (heterozygotes) and temca fin regenerates at 7 dpa shifted to 33 °C right after amputation (0 dpa shift) or at 2 dpa (2 dpa shift). (C) Quantification of fin regenerate lengths at 7 dpa (n = 12, 14, 12, and 11 for 0 dpa shift control and temca and for 2-dpa shift control and temca, respectively). (D) Representative EdU-staining images of control and temca fin regenerates at 2 dpa. (E) Quantification of EdU⁺ cells in blastema area of 2 dpa fin regenerates (n = 8). (F) Schematic showing amputation and imaging time points of tcfsiam:EGFP transgenic reporter line. (G) Representative whole-mount images of tcfsiam:EGFP control (heterozygotes) and temca fin regenerates at 24, 30, 36, 42, and 48 h postamputation (hpa) at 33 °C. (H) Quantification of tcfsiam:EGFP signal intensity (n = 10 and 9 for control and temca, respectively). Red arrowheads indicate amputation planes. Scale bars, 1 mm in B; 150 µm in D. Data are presented as mean ± SD. Student’s unpaired two-tailed t test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	scn8ab^pd291/pd291
Condition:	amputation: caudal fin, heat exposure
Observed In:	blastema cell population proliferation caudal fin caudal fin fin regeneration
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA