Fig. 3

(A-E) Acoustic startle (SLC) responsiveness of cacna2d3 mutants to the 10, low-level acoustic stimuli presented during the “sensitivity” phase of the habituation assay. Startle response of (A) wild-type (shown in blue) and cacna2d3^p177 mutants (shown in red). Startle response of (B) cacna2d3^sa16189 wild-type (shown in blue), heterozygous (shown in black) and mutant larvae (shown in red). Startle response of (C) cacna2d3^sa16051 wild-type (shown in blue), heterozygous (shown in black) and mutant larvae (shown in red). (B, C) ****p<0.0001, one-way ANOVA with Dunnett’s multiple comparison test. Startle response of (D) dory^p177/+ sibling larvae (shown in blue) and cacna2d3^p177/ cacna2d3^sa16189 trans-heterozygous larvae (shown in red). Startle response of dory^p177/+; siblings (shown in blue) and cacna2d3^p177/cacna2d3^sa16051 trans-heterozygous larvae (shown in red). (A, D, E) ****p<0.0001, unpaired t-test with Welch’s correction versus wild-type. (F) Startle frequency for 30 trials at 6 stimulus intensities. cacna2d3^sa16189/+ heterozygotes are in black and cacna2d3^sa16189 mutants are in red. (G) Mean startle sensitivity indices. ****p<0.0001, unpaired t-test with Welch’s correction versus heterozygotes. (H) Mean acoustic startle habituation percentage from a cross of cacna2d3^sa16051/+ heterozygotes and cacna2d3^sa16051 mutants with a lowered acoustic intensity. cacna2d3^sa16051/+ heterozygotes are shown in black, and homozygous cacna2d3^sa16051 mutants are shown in red. ****p<0.0001, unpaired t-test with Welch’s correction versus heterozygotes. For all panels, the number of larvae analyzed is shown below each bar. Error bars indicate SEM.