FIGURE

Fig. 4

ID

ZDB-FIG-220527-21

Publication

Xiao et al., 2022 - Identifying drivers of breast cancer metastasis in progressively invasive subpopulations of zebrafish-xenografted MDA-MB-231

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Fig. 4

Knock-down of DDIT4, MT1X, SERPINE1 and CTSD in MDA-MB-231?F2 cells revealed a role in breast cancer invasion. a qRT-PCR amplification quantifying knockdown of the respective gene within the MDA-MB-231?F2 population. Notably, expression of all genes was reduced to near parental expression levels. b After 24?hours, ECM embedded cell clusters of various knockdown and wildtype lines were quantified using ImageJ for invasion. Invasion is measured as relative area of cells that escape the cluster. c Representative immunofluorescence images (left panel of each pair) of embedded clusters stained for Hoechst (blue) and Phalloidin (red). Digitally zoomed insets taken from the full-sized image and highlighting invasion at the borders are provided (right panel of each pair). Insets correspond to dashed boxes on original scale image. Scale bars?=?100??m. *?=?p-value < .01; **?=?p-value < .001; ***?=?p-value < .0001

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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