FIGURE

Fig. 6

ID

ZDB-FIG-220520-35

Publication

Wang et al., 2022 - Essential Role of RIG-I in Hematopoietic Precursor Emergence in Primitive Hematopoiesis during Zebrafish Development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Rig-I knockdown has minimal effect on proliferation and apoptosis of hematopoietic cells

(A and B) Cell proliferation detected by whole-mount in situ immunohistochemistry using anti-pH3 Ab in 24-hpf embryos injected with control MO (A) or rig-I MO (B). (C and D) Cell apoptosis detected by TUNEL assay in 24-hpf embryos injected with control MO (C) or rig-I MO (D). The boxed areas are enlarged in the corresponding right panels. Images were captured under Olympus stereoscope (MVX10 MacroView; original magnification ×50). (E) Percentage of pH3-positive cells in embryos injected with a control or indicated morpholinos from (A) and (B). (F) Percentage of TUNEL-positive cells in embryos injected with a control or indicated morpholinos from (C) and (D). Each experiment was repeated three times (n = 3, mean ± SD, Student t test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-rigi
Knockdown Reagent:	MO2-rigi
Observed In:	intermediate cell mass of mesoderm apoptotic process intermediate cell mass of mesoderm cell population proliferation
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Immunohorizons