FIGURE

Fig. 4

ID

ZDB-FIG-220512-26

Publication

Phelps et al., 2022 - MITF deficiency accelerates GNAQ-driven uveal melanoma

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Fig. 4

CYSLTR2^L129Q-, YAP^AA-, and PLCβ4^D630Y-driven tumors do not display hyperactive ERK staining in the mitfa^−/− context. Genotype abbreviations are as described in Fig. 3. (A and B) Representative images of H&E staining and IHC for YAP, ERK, or phospho-ERK (pERK) on the indicated tumor genotypes. YAP activation is determined by nuclear localization and ERK activation by phospho-ERK. (A) C➛pm+ tumors and Qpm+ tumors are positive for nuclear YAP and phospho-ERK, while C➛pm−, Qpm−, and PLC➛pm− tumors display only YAP activation; n ≥ 3 for all genotypes and stains, except n = 2 for each Y➛pm− and C➛pm+ IHC stains. (B) Q➛Bpm− displays nuclear YAP but not phospho-ERK; n = 5 for all stains. (C) Graph shows the percent of UM tumor cells positive for phospho-ERK quantified by QuPath (n ≥ 3 for all genotypes, except n = 2 for Y➛pm− and C➛pm+), and error bars denote SD. Statistical significance is as follows: C➛pm+ versus C➛pm− P = 0.0435, Q➛pm+ versus Q➛pm− P = 0.0001, Y➛pm+ versus Y➛pm− P = 0.551, as determined by Student’s unpaired t test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA