FIGURE

Figure 7

ID

ZDB-FIG-220509-32

Publication

Shibata et al., 2022 - Abnormal male reproduction and embryonic development induced by downregulation of a phospholipid fatty acid-introducing enzyme Lpgat1 in zebrafish

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Figure 7

Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) species in sperm from lpgat1^+/− zebrafish. PC (A) and PE (B) species composition of zebrafish sperm was analyzed by LC–MS/MS. Semen was collected from an anesthetized zebrafish by abdominal compression. Sperm pellets were prepared from semen collected from one zebrafish and lipids were recovered. The numbers of sperm samples were n = 4 for lpgat1^+/+ and n = 4 for lpgat1^+/−. Error bars are S.D. Statistically significant differences are marked with asterisks. *p < 0.05; **p < 0.01; ***p < 0.001. Unpaired, two-tailed t test was used. For molecular species with low abundance, the relative abundance of each molecular species is shown in Supplementary Fig. S12.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lpgat1^zf3665/+ (AB) lpgat1^zf3666/+ (AB)
Observed In:	sperm phosphatidylcholine 32:0 sperm phosphatidylcholine 32:1 sperm phosphatidylcholine 34:0 sperm phosphatidylcholine 34:1 sperm phosphatidylcholine 34:2 sperm phosphatidylcholine 36:1 sperm phosphatidylcholine 36:2 sperm phosphatidylcholine 36:3 sperm phosphatidylcholine 36:4 sperm phosphatidylcholine 38:3 sperm phosphatidylcholine 38:4 sperm phosphatidylcholine 38:5 sperm phosphatidylcholine 38:6 sperm phosphatidylcholine 40:5 sperm phosphatidylcholine 40:6 sperm phosphatidylcholine 40:7 sperm phosphatidylethanolamine 34:1 sperm phosphatidylethanolamine 34:2 sperm phosphatidylethanolamine 36:1 sperm phosphatidylethanolamine 36:2 sperm phosphatidylethanolamine 36:3 sperm phosphatidylethanolamine 36:4 sperm phosphatidylethanolamine 38:3 sperm phosphatidylethanolamine 38:4 sperm phosphatidylethanolamine 38:5 sperm phosphatidylethanolamine 38:6 sperm phosphatidylethanolamine 40:5 sperm phosphatidylethanolamine 40:6 sperm phosphatidylethanolamine 40:7
Stage:	Adult

Phenotype Detail

Acknowledgments

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