Figure 2

Figure 2. Hematoxylin and eosin staining: (A) Control staining in uninjured retina (0 dpi) with distinct retinal layers. (B) 1 dpi: edema in the lesion area particularly affecting the IPL and INL. (C) 2 dpi: severe edema causing a major disruption to majority of the retinal layers. (D) 3 dpi: degeneration phase, coagulative necrosis involving all retinal layers and the formed ‘blister’. Low numbers of polymorphonuclear and mononuclear inflammatory cells were detected (arrow). (E) 5 dpi: remodeling phase, ‘blister’ is still present, expansion of and piling of retinal cells at the lesion periphery coincide with maximal proliferation levels (*). (F) 10 dpi: lesion size noticeably smaller in comparison to previous time points. (G) 20 dpi: lesion size reaches the thinnest point, clearing of dead cells complete. (H) 30 dpi: regrowth phase, largely regenerated retina, a clearly dysplastic region is evident exhibiting a mixture of retinal cells and absence of lamination. (I,J) 60 and 100 dpi: focal retinal reorganization, reduced number of rods and cones, inward growth of RPE, focal disruption and convergence of INL and ONL (circled). (K) 300 dpi: incomplete regeneration presenting the absence of photoreceptor outer segments (circled), dysplasia apparent with largely normal retina surrounding the affected area. Retinal layers labelled in control panel for reference; RPE—retinal pigment epithelium, ONL—outer nuclear layer, OPL—outer plexiform layer, INL—inner nuclear layer, IPL—inner plexiform layer, GCL—ganglion cell layer. 300 dpi at 40× with scale bar representing 100 µm to show the full regeneration process. Rest of scale bars represent 200 µm.