FIGURE

Figure 3

ID

ZDB-FIG-220426-74

Publication

Lane et al., 2021 - Steroid-sensitive nephrotic syndrome candidate gene CLVS1 regulates podocyte oxidative stress and endocytosis

Other Figures

All Figure Page

Back to All Figure Page

Figure 3

Loss of CLVS1 expression increases podocyte susceptibility to apoptosis that can be restored by corticosteroid treatment.

(A and B) Western blot showing significantly reduced expression of CLVS1 in CRISPR/Cas9-mediated knockout (KO) podocyte cell lines compared with control cell lines (n = 4, P < 0.001, 2-tailed t test). (C) Still images depicting cleaved caspase-3 activity (green) as reporter for early apoptosis and propidium iodide staining (red) for late apoptosis and necrosis in human podocytes 72 hours after serum starvation (scale bars = 1 mm). (D) Quantification of these images over time revealed an increase in podocyte susceptibility to serum starvation–induced apoptosis in CLVS1-KO and homozygous H310Y-knockin (KI) podocytes compared with controls (P < 0.05 for all time points after 16 hours for KO and after 34 hours for KI, n > 25 for each group, 2-way ANOVA). Heterozygous H310Y-KI podocytes displayed similar apoptotic phenotypes to controls. The elevated apoptosis in CLVS1-KO and homozygous H310Y-KI podocytes was rescued by treatment with 1 μM dexamethasone.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ JCI Insight