Knockdown dusp14 expression by morpholino decreases the number of hair cell and neuromasts in zebrafish. (A) Schematic diagram of zebrafish neuromast and inner ear hair cell. (B) Confocal imaging analysis of crista hair cells in the otic vesicle of control and dusp14 deficiency zebrafish at 72 hpf. (B′) The statistical analysis of the numbers of inner ear crista hair cells in the control and dusp14 morphants at 72 hpf. (B′′) The statistical analysis of the numbers of hair cells in the remaining neuromast L3 in the control and dusp14 morphants at 72 hpf. (C) The imaging analysis of control and dusp14 morphants at 72 hpf in fluorescent field. Scale bar = 500 μm. (C′) Quantification of the number of unilateral hair cell clusters of control and dusp14 morphants at 72 hpf. (D)in situ hybridization of the eya1 gene specifically expressed in the neuromasts showed that the number of neuromasts in dusp14-MO zebrafish was decreased. (D′) statistics results of D. Each bar represents the mean ± SEM. Values with *, ***, and **** above the bars are significantly different (P < 0.05, P < 0.001, and P < 0.0001, respectively).
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