FIGURE

FIGURE 5

ID

ZDB-FIG-220409-26

Publication

Subramaniam et al., 2022 - Coagulation Factor IIIa (f3a) Knockdown in Zebrafish Leads to Defective Angiogenesis and Mild Bleeding Phenotype

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FIGURE 5

f3a knockdown shows delayed angiogenesis: To study vascular development, endothelial-specific transgenic lines of zebrafish (flk1:egfp-NLS/kdrl:mCherry-CAAX) embryo were used to knockdown f3a. (A) Images represent MO-mediated knockdown of f3a expression resulting in morphological abnormalities. MO phenotypes: control MO injected (control MO), and mild, moderate, and severe phenotypes. Differences in caudal vein plexus (CVP) in the trunk regions are indicated by white arrows. (B) Schematic diagram depicts to identify Caudal vein (CV), caudal vein plexus (CVP) and Central aorta (CA). (C)Tg(fli1:Myr-mCherry) line was used to quantify CVP loop number and Sprouting number from the CVP at 48 hpf. Embryos were randomly picked from ∼60 MO-injected (6 from control and 9 from f3a MO) embryos from control and f3a.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y171Tg + MO1-f3a y171Tg; y7Tg + MO1-f3a
Knockdown Reagent:	MO1-f3a
Observed In:	caudal vein plexus caudal vein plexus sprouting angiogenesis central artery primordial midbrain channel vasculogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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