FIGURE

Figure 9

ID

ZDB-FIG-220403-44

Publication

Laghi et al., 2022 - Exploring Zebrafish Larvae as a COVID-19 Model: Probable Abortive SARS-CoV-2 Replication in the Swim Bladder

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Figure 9

Overexpression of hACE2-mCherry by plasmid injection at the 1-cell stage. (A) Verification of construct functionality by transfection in BHK cells. N mRNA levels measured 24 h after exposure of transfected cells with inactivated or with active SARS-CoV-2. (B) Optimization of the plasmid dose. Fluorescence intensity measured in 24-hpf embryos after injection at the 1-cell stage of the specified amount of the pz26hACE2-mCherryF plasmid together I-SceI, for the 25% embryos with the best expression in each group. The percentage of misshapen embryos in each group is indicated on the bottom of the graph. (C) representative image of a 24-hpf embryo (left) and a 3-dpf larva (right) after mock injection (top) or injection of 68 pg of pz26hACE2-mCherryF. (D) Representative flow cytometry analysis of cells dissociated from 4-dpf larvae, mock-injected (left) or injected with 68 pg of pz26hACE2-mCherryF (right). (E) Immunohistochemistry of a larva injected with 68 pg of pz26hACE2-2A-mCherryF, showing ACE2 detection of a mCherry-positive muscle fiber.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Infect Microbiol