Fig. 6

A Example of Treg recruitment to a necrotic R M. abscessus- wasabi granuloma in 14 dpi TgBAC(foxp3a:TagRFP,cryaa:EGFP)^vcc3 adult zebrafish. B Example of Treg recruitment to a necrotic S M. abscessus- wasabi granuloma from a 28 dpi TgBAC(foxp3a:TagRFP,cryaa:EGFP)^vcc3 adult zebrafish. Scale bars indicate 50 μm. M. abscessus-wasabi is coloured green, foxp3a:TagRFP positive Treg are marked in magenta. Images from A and B are representative of the dataset analysed in C. C Quantification of Treg RFP pixels as a function of M. abscessus-wasabi fluorescence in TgBAC(foxp3a:TagRFP,cryaa:EGFP)^vcc3 adult zebrafish. Each point represents a single lesion. Total n per group (lesions/animals): 14 dpi R = 26/4, S = 55/3; 28 dpi R = 66/3, S = 29/3. Statistical testing by 2-way ANOVA. D Enumeration of CFUs from foxp3a^vcc6 mutant adult zebrafish infected with 2 × 10⁴ CFU M. abscessus R. Each data point represents a single animal, data are representative of two biological replicates. Statistical testing by two-sided T test. Total number of animals: WT = 9, foxp3a−/− = 12. E Quantification of bacterial lesion necrosis in foxp3a^vcc6 mutant adult zebrafish infected with M. abscessus R. Each data point represents the proportion of lesions from a single animal. F Quantification of bacterial burden stratified by lesion necrosis in foxp3a^vcc6 mutant adult zebrafish infected with M. abscessus R. Each data point represents the proportion of lesions from a single animal. Total individual lesions and animals analysed in E, F (necrotic/unorganised/animals): WT (35/91/5); foxp3a^vcc6 mutant (22/97/4). Statistical testing by two-sided T test. Data are presented as mean values ± SD. Source data are provided as a Source Data file.