FIGURE

Fig. 4

ID

ZDB-FIG-220306-4

Publication

Elsaid et al., 2022 - Reduced α-galactosidase A activity in zebrafish (Danio rerio) mirrors distinct features of Fabry nephropathy phenotype

Other Figures

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Fig. 4

Histological distribution and enzymatic activity of Zebrafish- α-GAL protein in the GLA mutant zebrafish. (A) Scanned digital images show α-GAL antibody and periodic acid Schiff staining. Note that PAS staining shows dilated capillary loops and thinner Bowman's space (yellow arrowheads) in mutant compared to wild type animals. (B) Quantification of immunohistochemical analysis of sections from wild-type and mutant kidneys. Signal intensity is significantly higher in wild type compared to mutant tissues (Mann-Whitney U test: P < 0.05). The violin plot represents these results within the 95th percentile, and the dash-line symbol inside the violin plot represents the median. Scale bar = 50um. (C) Comparison of α-GAL activity in wild type and mutant zebrafish kidney tissue lysate (Mann-Whitney Test, P < 0.05).

Expression Data

Gene:	gla
Antibody:	Ab1-gla
Fish:	AB/TU gla^huh1/huh1 (AB/TU)
Anatomical Terms:	renal glomerulus renal tubule cytoplasm
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gla^huh1/huh1 (AB/TU)
Observed In:	capillary loop nephron kidney alpha-galactosidase activity renal capsular space renal glomerulus renal tubule cytoplasm
Stage:	Adult

Phenotype Detail

Acknowledgments

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