FIGURE
Figure 2
- ID
- ZDB-FIG-220302-216
- Publication
- Guo et al., 2022 - Multiplexed Genome Editing for Efficient Phenotypic Screening in Zebrafish
- Other Figures
- All Figure Page
- Back to All Figure Page
Figure 2
An overview of multiple-gene knockout technology and the generation of individual homozygous mutants. Cas9 mRNA and five gRNAs were synthesized and co-injected into 1–2 cell stage embryos of wild-type zebrafish. Mosaic F0 were in-crossed to generate mosaic F1 zebrafish. Then, F1 fish were out-crossed with wild-type to generate F2 fish. The gene mutation results of mosaic F1 were identified by sequencing. Heterozygous F3 zebrafish were crossed to generate homozygous mutants. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Vet Sci