FIGURE

Figure 4

ID

ZDB-FIG-220302-198

Publication

Trengove et al., 2022 - Functional Analysis of Zebrafish socs4a: Impacts on the Notochord and Sensory Function

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Figure 4

Mechanosensory analysis in socs4a knockdown embryos. Embryos injected with Ctl (A,A’,D,G), ATG (B,B’,E) or UTR (C,C’,H) MO were subjected to WISH with isl1a (A–C’) or neurod1 (D,E) at 26 hpf, or Mitotracker red (Mito) staining at 3 dpf (G,H) and imaged dorsally (A–E) and laterally (A’–C’,G,H), with the boxed areas highlighting the equivalent areas in the alternative images (A–C,A’–C’). Scale bars = 0.5 mm. Alternatively, the responsiveness to touch at 24 hpf (F) and water movement at 3 dpf (I) was quantified, with results presented for 3 independent experiments (n > 30 embryos in each group) along with mean ± SEM and statistical significance relative to Ctl (p < 0.05: *). Abbreviation: NM, neuromast.

Expression Data

Gene:	isl1a
Fish:	WT WT + MO1-socs4
Knockdown Reagent:	MO1-socs4
Anatomical Terms:	hindbrain trunk anterior region
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-socs4 WT + MO2-socs4
Knockdown Reagents:	MO1-socs4 MO2-socs4
Observed In:	hindbrain lateral line musculoskeletal movement, spinal reflex action neuromast sensory perception of touch trunk anterior region
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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