Overgrowth of endogenous Aeromonas spp. in tp53 mutants requires a sialidase activity. a, b A lethality curve of Aeromonas jandaei TP531 association (2 × 107 CFU/mL) with WT (a) or tp53 mutant (b) larvae upon the treatment of oseltamivir (10 μM) starting at 3 dpf. N = 60 each. The lethality curve was statistically estimated by Gehan-Breslow-Wilcoxon test. c Representative images showing that oseltamivir (1 μM) treatment dampened an elevated inflammatory response in tp53 mutants monitored by Tg(NFκB:EGFP). White dashed lines denote boundaries of GITs based on DIC bright field images. Scale bar = 50 μm. d A boxed plot of changes of NFκB dependent EGFP intensities in the mid-distal intestines of WT and tp53 mutants upon oseltamivir treatment. N = 12 each. e Representative pictures of bacterial cultures from WT or tp53 mutants using Aeromonas medium base with or without oseltamivir treatment. Aeromonas spp. was identified based on manufactural criteria (refer to Methods for details). Scale bar = 20 mm. f A bar graph showing that the dramatically increased CFU numbers of Aeromonas spp. in tp53 mutants were abrogated upon treatment of oseltamivir (1 μM). The boxed plot and the bar graph were statistically estimated by non-parametric Friedman test followed by Dunn’s multiple comparisons test. Data are represented as mean ± SEM. N = 20 each. *< p = 0.05; **< p = 0.01; ****< p = 0.001. NS not significant
|
