FIGURE

Figure 4

ID

ZDB-FIG-211219-71

Publication

Rondelli et al., 2021 - The ubiquitous mitochondrial protein unfoldase CLPX regulates erythroid heme synthesis by control of iron utilization and heme synthesis enzyme activation and turnover

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Figure 4

CLPX regulates the terminal steps of heme synthesis.A, ALAS2 activity was significantly increased in Clpx^−/− and Clpp^−/− cells. B, Clpx^−/− and Clpp^−/− cells produced significantly more ALA than WT cells. C, Clpx^−/− and Clpp^−/− cells produced significantly more PPIX than WT cells. D, PPOX protein levels were decreased in undifferentiated Clpx^−/− and Clpp^−/− cells but not appreciably in differentiated cells. However, FECH protein levels were decreased, N = 3. E, PPOX activity was decreased in Clpx^−/− cells but was unaltered in Clpp^−/− cells. F, FECH activity was decreased in undifferentiated Clpx^−/− cells, relative to wild-type cells. The FECH activity of Clpp^−/− cells was unaltered. In differentiating MEL cells, FECH activity was decreased in both Clpx^−/− and Clpp^−/− cells. ∗p < 0.05. Error bars indicate SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Biol. Chem.