FIGURE

Figure 7

ID
ZDB-FIG-211216-153
Publication
Dalum et al., 2021 - High-Resolution, 3D Imaging of the Zebrafish Gill-Associated Lymphoid Tissue (GIALT) Reveals a Novel Lymphoid Structure, the Amphibranchial Lymphoid Tissue
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Figure 7

The ILT and ALT are structured by a complex network of reticulated epithelial cells. Representative deconvolved confocal images of adult zebrafish gills displaying the ILT (A) and the ALT (B) at high-magnification. The localization of sections (A, B) is illustrated on panels (C, D). The images were acquired from 30 μm whole-body cryosections stained with phalloidin (green) and DAPI (blue) and reticulated epithelial cells were labeled with an anti-cytokeratin antibody (red hot). While the cytokeratin signal is strong within the outermost layer of the epithelium, an intricate core of cytokeratin positive cells is observed within ILT (A) and ALT (B). Images are maximum intensity projections (2 µm). Annotations: Aa, Afferent artery; ALT, Amphibranchial Lymphoid Tissue; Bc, Branchial cavity; C, Cartilage; Ea, Efferent artery; Ga, Gill arch; ILT, Interbranchial Lymphoid Tissue; La, Lamella and S, Septum. Scale bars: 20 μm.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol