FIGURE

Figure 5.

ID

ZDB-FIG-211120-11

Publication

Gui et al., 2021 - De novo identification of mammalian ciliary motility proteins using cryo-EM

Other Figures

All Figure Page

Back to All Figure Page

Figure 5.

Pierce1 and Pierce2 link the ODA-DC to the MIP architecture

(A) Atomic model showing Pierce1 and Pierce2 spanning the microtubule wall (shown in transparent surface representation) and linking the external ODA-DC to the filamentous MIP CFAP53.

(B and C) Interaction of the Pierce1 (B) and Pierce2 (C) N termini with CFAP53.

(D) Superposition of Pierce1 and Pierce2. A conserved central region interacts with the CCDC114/151 coiled coil. The sequence alignment of this conserved region is shown beneath.

(E) Expanded view showing a potential hydrogen bond between two neighboring CFAP53 molecules involving R158 and Q495. Mutation of R158 to glycine has been implicated casually in dextrocardia (Noël et al., 2016).

See also Video S1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Cell, 184(23), Gui, M., Farley, H., Anujan, P., Anderson, J.R., Maxwell, D.W., Whitchurch, J.B., Botsch, J.J., Qiu, T., Meleppattu, S., Singh, S.K., Zhang, Q., Thompson, J., Lucas, J.S., Bingle, C.D., Norris, D.P., Roy, S., Brown, A., De novo identification of mammalian ciliary motility proteins using cryo-EM, 5791-5806.e19, Copyright (2021) with permission from Elsevier. Full text @ Cell