FIGURE

Figure 4.

ID

ZDB-FIG-211111-42

Publication

Jin et al., 2021 - egfl6 expression in the pharyngeal pouch is dispensable for craniofacial development

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Figure 4.

Expression of egfl7 in the pharyngeal region. (A, B) Fluorescence in situ hybridization of egfl7 (green) in conjunction with the GFP immunohistochemistry (red) in wild-type animals at 30 hpf. (A) egfl7 is expressed segmentally in small patches (arrows) adjacent to her5-positive pouches (1-4). (B) egfl7 expressing small patches (arrows) are located at the ventral tip of sox10-positive pharyngeal arches (PA2-4) but rarely overlapped with PAs. (C-E) Alcama immunohistochemistry (green) labels five pouches (1-5) in wild-type (n = 92), egfl7-MO (n = 80), and egfl7-MO-injected egfl6 mutant (n = 21) embryos at 34 hpf. Sensory ganglia are indicated with asterisks. (F-H) At 4 dpf, rag1 expression (green) in the thymus is normal in wild-type (n = 74), egfl7-MO (n = 76), and egfl7-MO-injected egfl6 mutant (n = 14) zebrafish. (I-K) Facial cartilages, including the HM and CBs, are normal in wild-type (n = 105), egfl7-MO (n = 84), and egfl7-MO-injected egfl6 mutant (n = 19) animals at 5 dpf. Scale bar: 40 μm.

Expression Data

Gene:	egfl7
Fish:	ba2Tg ne1911Tg
Anatomical Term:	pharynx
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO1-egfl7 egfl6^{zf3831/zf3831} + MO1-egfl7 (TU)
Knockdown Reagent:	MO1-egfl7
Observed In:	ceratobranchial cartilage cranial skeletal system development hyosymplectic cartilage pharyngeal system development
Stage Range:	Prim-15 to Day 4

Phenotype Detail

Acknowledgments

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