FIGURE

Figure 4

ID

ZDB-FIG-211103-74

Publication

Holmgren et al., 2021 - Mechanical overstimulation causes acute injury and synapse loss followed by fast recovery in lateral-line neuromasts of larval zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Figure 4

Significant hair-cell synapse loss is observed in ‘normal’ neuromasts following mechanical overstimulation and exacerbated by blocking glutamate uptake.

(A–C) Representative maximum intensity projection images of unexposed (A), or stimulus exposed lateral-line neuromast with ‘normal’ (B) or ‘disrupted’ (C) morphology. Synaptic ribbons (magenta; Ribeye b), PSDs (green; MAGUK) and hair cells (blue; Parvalbumin) were immunolabled. Scale bar: 5 µm (D–E) Intact synapses per neuromast hair cell. Pink box plot in D (Exp) represents pooled exposed neuromasts while, in E, gray (Norm) and red (Dis) plots represent neuromasts parsed into normal and disrupted groups. Whiskers = min to max. The average number of intact synapses per hair cell was significantly reduced in exposed neuromasts (D; **Adj p = 0.0078); when parsed, this reduction was significant in the ‘normal’ exposure group relative to control (E; **Adj p = 0.0043 normal, Adj p = 0.1207 disrupted). (F) Differences of least squares means in number of intact synapses per hair cell between groups. Bars represent 95% CI. (G) The number of intact synapses per hair cell in larvae co-treated with TBOA, to block glutamate clearance, or drug carrier alone during exposure. Synapse loss was significantly greater in ‘normal’ neuromasts co-exposed to TBOA compared to fish co-exposed to the drug carrier alone (Two-way ANOVA. *p < 0.0187).

Expression Data

Antibodies:	Ab1-pan-MAGUK Ab3-ctbp2l Ab6-pvalb
Fish:	AB
Conditions:	mechanical stress chemical treatment by environment: vesicular glutamate transport inhibitor, mechanical stress
Anatomical Terms:	neuromast hair cell neuromast hair cell postsynaptic density neuromast hair cell synaptic ribbon
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB
Conditions:	mechanical stress chemical treatment by environment: vesicular glutamate transport inhibitor, mechanical stress
Observed In:	neuromast hair cell neuromast hair cell postsynaptic density neuromast hair cell synaptic ribbon
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife