FIGURE

Figure 2.

ID

ZDB-FIG-211014-3

Publication

Jha et al., 2021 - High behavioural variability mediated by altered neuronal excitability in auts2 mutant zebrafish

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Figure 2.

Morphologic characterization of auts2a mutants. A, Bright field images of wild-type (WT) and mutant (MUT) larvae. Scale bar: 2 mm. B, Whole-mount immunostaining with 3A10 antibody at 30 hpf in auts2a mutants. Arrowhead points to the cell body of the Mauthner neuron and arrow points to the axon. C, Maximum intensity Z-projection of Mauther neuron (top) and homologs (bottom) of wild-type (left) and mutant (right) larvae. Scale bar: 10 μm. D, Comparison of Mauthner lateral dendrite lengths in wild-type and auts2a mutant larvae. E, Comparison of Mauthner soma volume in WT and mutant larvae. n_WT = 8 cells from 7 larvae; n_mut = 15 cells from 12 larvae; *p < 0.05; ns: not significant; Mann–Whitney U test. F, Comparison of soma volume of the M-cell homologs. n_WT = 7 cells from 5 larvae; n_mut = 14 cells from 12 larvae. ns, not significant; Mann–Whitney U test.

Expression Data

Antibody:	Ab-3A10
Fish:	AB auts2a^{ncb104/ncb104} (AB)
Anatomical Terms:	Mauthner neuron dendrite Mauthner neuron neuronal cell body
Stage:	Prim-15

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	auts2a^{ncb104/ncb104} (AB)
Observed In:	Mauthner neuron dendrite Mauthner neuron neuronal cell body whole organism
Stage Range:	Prim-15 to Day 5

Phenotype Detail

Acknowledgments

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