FIGURE

Figure 4

ID

ZDB-FIG-211002-119

Publication

Vecchi et al., 2021 - Generation of a Triadin KnockOut Syndrome Zebrafish Model

Other Figures

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Figure 4

Heart morphology and function evaluation. (A,B) Frontal view of 48 hpf std-MO and trdn-MOs embryos hybridized with cmlc2 probe using WISH technique. Scale bars indicate 100 μm. (C,D) Semithin section of std-MO and trdn-MOs embryos in the heart region. Scale bars indicate 100 μm. (E) Quantification of heartrate in embryos at 48 hpf injected with std-MO and trdn-MOs. (F) Percentage of embryos with a heartbeat superior to the mean of the heartbeat (bpm) of trdn-MOs. (G) Heartbeat count in 3 dpf std-MO and trdn-MOs untreated or adrenaline-, isoprenaline- and atropine-treated embryos. (H) Heartbeat count in 3 dpf std-MO and trdn-MOs untreated or treated with metoprolol or flecainide. (F–H) At least 30 embryos were analyzed for each group. Values are expressed as mean ± SEM. *** p < 0.001, ** p < 0.01, n.s. = not significant, Mann–Whitney test (E) and one-way ANOVA followed by Tukey post-hoc correction (G,H). Adr, adrenaline; Iso, isoprenaline; Atr, atropine.

Expression Data

Gene:	myl7
Fish:	WT WT + MO2-trdn + MO3-trdn
Knockdown Reagents:	MO2-trdn MO3-trdn
Anatomical Term:	heart
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO2-trdn + MO3-trdn
Conditions:	chemical treatment by environment: adrenaline chemical treatment by environment: atropine chemical treatment by environment: flecainide chemical treatment by environment: isoprenaline chemical treatment by environment: metoprolol
Knockdown Reagents:	MO2-trdn MO3-trdn
Observed In:	heart heart contraction
Stage Range:	Long-pec to Protruding-mouth

Phenotype Detail

Acknowledgments

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