FIGURE

Figure 7.

ID

ZDB-FIG-210927-50

Publication

Wilson et al., 2021 - Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

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Figure 7.

EGFP-Plin2 decorates lipid droplets in cells surrounding neuromasts.

(A) Cartoon of 15 days of post fertilization (dpf) larval zebrafish showing the general location of lateral line neuromast images shown in panel B. The standard length of larvae imaged at this stage was 5.14 ± 0.26 mm (mean ± SD, n = 47 fish, see Figure 6—source data 1). (B) Examples of lipid droplets around neuromasts in fish heterozygous for Fus(EGFP-plin2) at 15 dpf. Fish were fed Gemma for 10 days and then stained with LipidTOX Red for a minimum of 2 hr prior to imaging. Insets show enlarged images of the lipid droplets in the boxed regions. Scale = 10 µm, standard length of the fish shown is noted in the brightfield image panel. Images are representative of at least 10 fish from three independent clutches.

Expression Data

Gene:	EGFP
Fish:	plin2^c874Tg/+
Anatomical Term:	neuromast lipid droplet
Stage:	Days 14-20

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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