(A and B) WISH showing the spatial expression of fabp2 at 2 (A) and 4 (B) dpf. (C?E) Confocal images at 4 dpf of WT (C), apoBa?/? apoBb.1MO (D), and mtpMO (E) embryos in the TgBAC(cldn15la-GFP)pd1034 reporter background, stained with phalloidin (red). Enlarged lumen caliber (arrow) is detected in apoBa?/? apoBb.1MO and mtpMO embryos, as quantified (F) (n = 2, nWT = 17, napoBa?/? apoBb.1MO = 15, nmtpMO = 16). The data are shown as the mean ± SEM, calculated using ANOVA followed by Tukey?s multiple-comparison test. *P < 0.05. (G and H) Confocal images of the guts of 4 dpf apoBa?/? and apoBa?/? apoBb.1MO embryos stained with Nile red, show no lipid accumulation. (I and J) Bright-field images of 5 dpf embryos stained with Alcian blue showing reduced number of goblet cells in apoBa?/? apoBb.1?/? double mutants (J) as compared with WT embryos (I), as quantified (K) (n = 3, nWT = 30, napoBa?/? apoBb.1?/? = 27, napoBa?/? apoBb.1MO = 27). The data are shown as the mean ± SEM, calculated using ANOVA followed by Tukey?s multiple-comparison test. ****P < 0.0001. (L) Confocal images at 5 dpf of WT and apoBa?/? apoBb.1MO embryos in the Tg(12xNRE:Egfp) reporter background. (M) Quantification of number of cells displaying active Notch signaling in the guts of WT and apoBa?/? apoBb.1MO embryos. The data are shown as the mean ± SEM, calculated using 2-tailed Student?s t test. Scale bar: (A?E, G, and H) 50 ?m, (I, J, and L) 100 ?m. **P < 0.01. P < 0.05 (considered significant versus control group).
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