FIGURE

FIGURE 2

ID

ZDB-FIG-210902-26

Publication

Koh et al., 2021 - Fluorescence Correlation Spectroscopy Reveals Survival Motor Neuron Oligomerization but No Active Transport in Motor Axons of a Zebrafish Model for Spinal Muscular Atrophy

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FIGURE 2

Expression and diffusion coefficients of FPs and FP-tagged Smn in zebrafish motor neurons. (A) Expression of eGFP, eGFP-Smn, mCherry, and mCherry-Smn in cell body and axon of motor neurons at 2 dpf. Images of cell body and axon are taken from different motor neurons. Scale bar = 5 μm. Red arrow indicates representative position where FCS measurement was taken in axon. (B) Average diffusion coefficients, D₁ (μm²/s) of eGFP, eGFP-Smn, mCherry, mCherry-Smn in cell body. (C) Average diffusion coefficients, D₂ (μm²/s) of eGFP-Smn, mCherry-Smn in cell body. (D) Average diffusion coefficients, D₁ (μm²/s) of eGFP, eGFP-Smn, mCherry, mCherry-Smn in axons. (E) Average diffusion coefficients, D₂ (μm²/s) of eGFP-Smn, mCherry-Smn in axons. Error bars represent the SD. (^n.s.P > 0.05, *P < 0.05). The numbers above the graph indicate the numbers of fish (number of points) the measurements were taken in.

Expression Data

Gene:	mCherry
Fish:	ns12Tg/ns12Tg
Anatomical Term:	motor neuron
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ns12Tg/ns12Tg
Observed In:	neuron protein transport neuron regulation of protein oligomerization
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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