FIGURE

Figure 4

ID

ZDB-FIG-210902-144

Publication

Sarkar et al., 2021 - Involvement of Oxidative and Endoplasmic Reticulum Stress in RDH12-Related Retinopathies

Other Figures

All Figure Page

Back to All Figure Page

Figure 4

Generation and characterisation of rdh12^u533 mutant zebrafish. (A) Sanger sequencing traces showed a 7 bp deletion (c.17_23del) in the rdh12^u533 mutant fish. The deleted bases are highlighted in blue on the wildtype trace. (B) RT-qPCR showed a significant reduction of rdh12 mRNA expression in the rdh12^u533 fish at 5 dpf (** p ≤ 0.001 analysed by paired t-test). (C,D) Retinal sections from 12 mpf fish were stained with toluidine blue to assess retinal structure. (E,F) TUNEL assay revealed no cell death Immunohistochemistry staining was used to detect rhodopsin (red) (G,H), blue opsin (green) (I,J), and red/green opsin (green) (K,L). Sections were counterstained with DAPI (blue). Rhodopsin mislocalisation was observed in rdh12^u533 fish. Scale bar = 50 µM. IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; IS, inner segment; OS, outer segment; RPE, retinal pigment epithelium.

Expression Data

Genes:	rdh12 rho
Fish:	AB rdh12^{zf3693/zf3693}
Anatomical Terms:	eye photoreceptor cell photoreceptor inner segment eye photoreceptor cell photoreceptor outer segment retinal outer nuclear layer whole organism
Stage Range:	Day 5 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rdh12^{zf3693/zf3693}
Observed In:	eye photoreceptor cell photoreceptor inner segment retina cell death retinal outer nuclear layer whole organism
Stage Range:	Day 5 to Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.