FIGURE

FIGURE 2

ID

ZDB-FIG-210828-9

Publication

Islam et al., 2021 - The Zebrafish Meiotic Cohesin Complex Protein Smc1b Is Required for Key Events in Meiotic Prophase I

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FIGURE 2

Smc1b mutant zebrafish display defects in spermatogenesis. (A)Smc1b mutations used in this study: smc1b^Q198X located in exon 4 and smc1b^Q261X in exon 5. Both mutations cause premature termination codons. (B–E) Hematoxylin-eosin (H&E) staining on adult testes of wild type (B), smc1b^{Q261X(–/–)}(C), smc1b^{Q198X(–/–)}(D), and smc1b^Q198X/Q261X(E). Dashed lines denote examples of: spermatogonia (yellow), spermatocytes (red), and spermatozoa (orange). (F,G) Caspase-3 (green) and DAPI (blue) staining on adult testes from wild type (F) and smc1b mutant (G). Caspase-3 positive cells are indicated by arrows. (H) Quantification of caspase-3 positive cells per testis area in the mutant (N = 3 testes) and wild type (N = 3 testes). Student’s t-test shows no significant (p = 0.3524) difference between wild-type and mutant caspase-3 positive cells numbers. Scale bars are 50 μm.

Expression Data

Antibody:	Ab3-casp3
Fish:	TU smc1b^{sa24632/sa24632} (TU)
Anatomical Term:	testis
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smc1b^{sa24631/sa24631} (TU) smc1b^{sa24632/sa24632} (TU) smc1b^sa24631/+; smc1b^sa24632/+ (TU)
Observed In:	sperm spermatocyte spermatogonium testis apoptotic process
Stage:	Adult

Phenotype Detail

Acknowledgments

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