Fig. 1

a Confocal imaging in the CHT of 48 hpf kdrl:mCherry/cmyb:GFP embryos. Embryos non-treated (NT) or incubated with H₂O₂ (3 mM). b Quantification of HSPCs associated with ECs. Statistical analysis: unpaired two-tailed t test, ***P < 0.001. Center values denote the mean, and error values denote s.e.m. c Anti-GFP and pH3 immunostaining of NT or H₂O₂-treated (3 mM) cmyb:GFP embryos. d Quantification of proliferating HSPCs; statistical analysis: unpaired two-tailed t test, ***P < 0.001. Center values denote the mean, and error values denote s.e.m. ecmyb expression at 48 hpf in NT embryos, and embryos treated with H₂O₂ (3 mM) or GSH (10 µm). f Quantification of cmyb-expressing cells in the CHT at 48 hpf. Statistical analysis was performed using a one-way ANOVA with Tukey–Kramer post hoc tests, adjusted for multiple comparison, ****P < 0.0001; (n.s.) non-significant P = 0.15. Center values denote the mean, and error values denote s.e.m. Scale bars: 50 μm (a); 25 μm (c); 100 μm (e).