FIGURE

Fig 8

ID

ZDB-FIG-210802-21

Publication

Wen et al., 2021 - The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics

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Fig 8

Knockdown of <italic toggle='yes'>tagln2</italic> impairs junction remodeling and rearrangement of F-actin cytoskeleton in CV pruning.

(A) and (B) Time-lapse live imaging of junction remodeling and actin cytoskeleton dynamics in CV prunig in Tg(Fliep:Lifeact-EGFP);KI(cdh5-mRFP) embryos. (A) Control embryo shows a detachment of junction (blue arrowheads) and an opposite direction of junction movement (white arrowheads) as multicellular tube became stenosis. F-actin forms at cdh5-positive junction (white and blue arrowheads), and undergoes a similar rearrangement as cdh5-positve junctions. Seven vascular loops are taken time-lapse live imaging at the stage of multicellular tube. Scale bar: 25 μm. (B) In tagln2 morphant, cdh5-positive junction moves to the right and connected with the adjacent one (white arrowheads), and F-actin undergoes similar rearrangement within it (white arrowheads). However, depolymerization of F-actin is also observed at junctions (white arrowheads). Six vascular loops are taken time-lapse live imaging. Scale bar: 20 μm.

Expression Data

Genes:	EGFP mRFP
Fish:	cdh5^cqu2Tg; cqu6Tg cdh5^cqu2Tg; cqu6Tg + MO2-tagln2
Knockdown Reagent:	MO2-tagln2
Anatomical Terms:	caudal vein plexus cell-cell junction caudal vein plexus filamentous actin
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdh5^cqu2Tg; cqu6Tg + MO2-tagln2
Knockdown Reagent:	MO2-tagln2
Observed In:	caudal vein plexus actin cytoskeleton organization caudal vein plexus blood vessel remodeling caudal vein plexus cell-cell junction maintenance
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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