Fig. 5

Rab5C controls Vegf and Notch signaling in vivo. a Western blot analysis of zebrafish lysates probed for Vegfr2 with α-actin as a loading control. A representative blot from n = 3 is shown. b Quantification of Western blots showing relative Vegfr2 levels (normalized to α-actin). Levels in embryos injected with Control MO were set to 1. Results are means + SEM (n = 3), 10 embryos were used per condition per experiment. AU, arbitrary units. c Inverted maximum projections of confocal z-stacks acquired by time-lapse microscopy in Tg(fli1a:lifeact-EGFP)^mu240 zebrafish embryos injected with Control MO (left) or rab5c MO (right). Scale bar, 10 μm. DA, dorsal aorta. dTg(TP1:Venus-PEST);(kdrl-mCherry)^s896 zebrafish embryos demonstrating activation of Notch signaling in embryos injected with Control MOs (top), which is reduced in embryos injected with rab5c MOs (bottom). Scale bar, 20 μm