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(A) Body length measured after diethylstilbestrol (DES) exposure at 25 dpf, 45 dpf, and 60 dpf, respectively. (B) Body length and body weight of zebrafish maintained after exposure, measured at 90 dpf. Different shapes of dots are body weight, and histogram is the body length (yellow is initial development stage (IDS), blue is gonadal differentiation stage (GDS), and pink is gonadal maturity stage (GMS)). (C) The plumpness (K) of zebrafish maintained after exposure, measured at 90 dpf. Values are shown as the mean ± SD (n = 3 replicates per treatment, n = 11 fish per replicate at 25, 45, and 60 dpf, and n = 15 fish per replicate at 90 dpf). Asterisks denote significant differences between control and treatments (* p < 0.05, ** p < 0.01, *** p < 0.005, and **** p < 0.0001, according to Dunnett’s multiple comparisons test). CK-control group.
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