FIGURE

Figure 2.

ID

ZDB-FIG-210708-68

Publication

Pollock et al., 2021 - Transcriptomic analysis of zebrafish prion protein mutants supports conserved cross-species function of the cellular prion protein

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Figure 2.

RNA-Sequencing show 1249 genes with an increase or decrease of log₂ fold change of 0.5 between wild-type and compound homozygous prion mutant zebrafish larvae. (A) Scatter graph showing relative FPKM values for wild-type (X-axis) and mutant (Y-axis) genes after RNA-sequencing. (B) Methodology diagram showing RNA-sequencing workflow. Two groups, wild-type and prion mutant (prp1^{ua5003/ua5003}; prp2^{ua5001/ua5001}) homozygous fish, each with three replicates containing a pool of 50 3dpf larvae were processed and sent for RNA-sequencing. Heatmap displays sample of the 25 genes with a biggest differential abundance in FPKM in wild-types compared to mutants. (C) Relative transcript abundance between wild-type and prion mutant fish comparing prp1 and prp2 through both RNA-sequencing and RT-qPCR analysis. D) Relative transcript abundance of ncam1a and st8sia2 through both RNA-sequencing and RT-qPCR analysis. * = P < 0.05

Expression Data

Genes:	ncam1a prnpb prnprs3 st8sia2
Fish:	prnpb^{ua5003/ua5003}; prnprs3^{ua5001/ua5001}
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	prnpb^{ua5003/ua5003}; prnprs3^{ua5001/ua5001}
Observed In:	whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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