Figure 4

Analysis of social preference in Neu1-KO and WT zebrafish using the 3-chambers test. (a–g) Analysis of the 3-chambers test with 4 unfamiliar zebrafish in Neu1-KO and WT zebrafish. (a) Apparatus of the 3-chambers test using 4 zebrafish. (b) Tracking of WT and Neu1-KO zebrafish. (c) Total swimming distance. (d) Time spent in the fish chamber area. (e) Total entry into the fish chamber area. (f) Time spent in the empty chamber area. (g) Total entry into the empty chamber area. n = 8 for each group. (h–o) Analysis of the 3-chambers test with 4 cavefish in Neu1-KO and WT zebrafish. (h) Apparatus of the 3-chambers test with 4 cavefish. (i) Tracking of WT and Neu1-KO zebrafish. (j) Total swimming distance. (k) Swimming speed. (l) Time spent in the fish chamber area. (m) Total entry into fish chamber area. (n) Time spent in the empty chamber area. (o) Total entry into empty chamber area. n = 10 for each group. Results are shown as means ± standard deviation. All values were compared using Mann–Whitney U test. n.s., not significant.