FIGURE

Figure 4

ID

ZDB-FIG-210424-4

Publication

Inoue et al., 2021 - Leucyl-tRNA synthetase deficiency systemically induces excessive autophagy in zebrafish

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Figure 4

Immunoelectron microscopy of larsb-knockout larvae under Tg[fabp10:mcherry] background. (A–C) Immunoelectron microscopy of the liver, skeletal muscle, and spinal cord of larsb^+/+ larva. (D–I) Immunoelectron microscopy of the liver, skeletal muscle, and spinal cord of larsb^−/− larva. The bottom row shows higher magnification images (G–I). Large vacuoles in the livers of larsb^−/− larvae (asterisks) were composed of numerous irregular membranous structures, which showed immunoreactivity against both Lc3b (black arrows) and mCherry (black arrowheads) (D,G). Scale bar: 5.0 µm for (A,D); 1.0 µm for B and E; 500 nm for (C,F,H); 100 nm for (G,I). AP: autophagosome, N: nucleus, Larsb: leucyl-tRNA synthetase, Lc3b: microtubule-associated protein 1A/1B-light chain 3.

Expression Data

Antibody:	Ab3-map1lc3b
Fish:	oi2Tg (AB) lars1b^oi3/oi3; oi2Tg (AB)
Anatomical Terms:	liver liver autolysosome liver autophagosome skeletal muscle skeletal muscle autolysosome skeletal muscle autophagosome spinal cord spinal cord autolysosome spinal cord autophagosome
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lars1b^oi3/oi3; oi2Tg (AB)
Observed In:	liver autolysosome liver autophagosome skeletal muscle autolysosome skeletal muscle autophagosome spinal cord autolysosome spinal cord autophagosome
Stage:	Day 6

Phenotype Detail

Acknowledgments

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