GCRV or poly(I:C) increases the expression of bmp8a.a Expression of bmp8a mRNA in ZFL cells after infected with GCRV (5 × 104 TCID50 per ml). b–e Expression of bmp8a mRNA in the liver (b), spleen (c), intestine (d), and kidney (e) from zebrafish injected i.p. with poly(I:C) (10 μg/fish). f–i Expression of bmp8a mRNA in the liver (f), spleen (g), intestine (h), and kidney (i) from zebrafish injected i.p. with 50 µl of GCRV (108 TCID50 per ml). Zebrafish injected i.p. with PBS or MEM were used as the control. The expression of actb1 served as a control for the qRT-PCR. Data were from three independent experiments and were analyzed by Student’s t-test (two-tailed). All data were presented as mean ± SD (*p < 0.05, **p < 0.01, and ***p < 0.001, ns means no significant difference).
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