FIGURE

Fig. 4

ID

ZDB-FIG-210326-4

Publication

Koopman et al., 2021 - The zebrafish grime mutant uncovers an evolutionarily conserved role for Tmem161b in the control of cardiac rhythm

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Fig. 4

Tmem161b expression is specific to excitable cells and localizes to plasma membranes. A tmem161b BAC transgenic line, Tg(tmem161b^BAC:tmem161b-citrine), generated by fusing a Citrine cassette to the C terminus of the Tmem161b protein, permits analysis of the tmem161b expression pattern as well as protein localization. (A) At 5 dpf, Tg(tmem161b^BAC:tmem161b-citrine) transgenics show Tmem161b-Citrine expression in excitable cells, including cardiomyocytes (bracket), ionocytes (arrows), and neuromasts (arrowheads). (B) High-magnification view of ventricular cardiomyocytes at 5 dpf of Tg(tmem161b^BAC:tmem161b-citrine) embryos crossed to the Tg(myl7:cherry-caax) line (cardiomyocyte reporter) shows Tmem161b-Citrine localization at or near the plasma membrane of cardiomyocytes. (C) Imaging of ionocytes and neuromasts of Tg(tmem161b^BAC:tmem161b-citrine) at 5 dpf showing enrichment for Tmem161b-Citrine in these cell types. Ionocyte and neuromast identity are confirmed by staining with Mitotracker (magenta).

Expression Data

Genes:	Citrine mCherry
Fish:	uq24ksTg bns7Tg; uq24ksTg
Anatomical Terms:	cardiac muscle cell cardiac muscle cell plasma membrane ionocyte neuromast
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA