Fig. 6

Zisp overexpression mimics noggin overexpression activity and acts upstream of the bone morphogenetic protein (BMP) receptor. a Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis of mRNA levels of 24 known palmitoyl acyltransferases in propofol-treated retinas (5 μg/ml propofol). Ef1a was used as loading control. n = 3. b Injection of zisp mRNA induced dorsalization phenotypes, including an up-turned tail (arrow), a reduction of tail fin (arrowheads), and a reduction of tail and trunk. n = 13. Scale bar, 15 mm. c Zisp inhibited ventralization phenotypes by BMP ligands (bmp2b and bmp7) but not by BMP receptors (bmpr1a and bmpr1b) or Smad1. Numbers indicate the number of embryos scored. n = 3. d Zisp inhibited bmp2b- and bmp7-induced eve1 expression. ef1α was used as an RT-PCR control. n = 3. e–h Following zisp overexpression, the eve1 and gata2 expression domains are greatly reduced (e, f), whereas the shh and gsc territories appear to have expanded (g, h). n = 17. i–k Following zisp overexpression, bmp2b expression is still present at the blastula stage (j), whereas it is lost in the ventral blastoderm (arrowheads) and maintained in the marginal zone (arrows) (k). e–h, j Animal pole views. k Lateral views. Scale bar, 75 μm