FIGURE

Figure 5

ID

ZDB-FIG-210310-69

Publication

Lu et al., 2021 - Generation of Knockout and Transgenic Zebrafish to Characterize Abcc4 Functions in Detoxification and Efflux of Lead

Other Figures

All Figure Page

Back to All Figure Page

Figure 5

Expression of foreign Abcc4 in transgenic zebrafish. (A) A primer pair (Flag-F and ABC-R3) was designed to recognize the abcc4 coding sequence (CDS) and Flag sequence. (B) Positive F₂ fish were individually crossed with WT fish to obtain positive F₃ embryos. Sixty larvae at 96 hpf were pooled for RNA extraction and subsequent RT-PCR analysis with a primer pair (Flag-F and ABC-R3). WT: Wild-type zebrafish embryos at the same developmental stage. F₃ abcc4-1# and F₃ abcc4-2# indicated the pooled larval zebrafish at 96 hpf from two positive F₂ zebrafish, which were individually crossed with WT fish, respectively. (C) qPCR analysis for abcc4 mRNA levels with a primer pair (abcc4–qPCR-F and abcc4–qPCR-R). (D) Western blotting analysis of Flag-tagged Abcc4 expression in F₃ embryos. (E) WISH analysis of abcc4 expression in WT and transgenic zebrafish larvae. Arrow heads indicate the brain and intestine.

Expression Data

Gene:	abcc4
Fish:	ihb367Tg/+ (AB)
Anatomical Terms:	brain intestine whole organism
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ihb367Tg/+ (AB)
Observed In:	brain intestine whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.