FIGURE

FIGURE 2

ID

ZDB-FIG-210310-25

Publication

Ranawakage et al., 2021 - Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor

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FIGURE 2

Cleavage efficiency of the selected crRNA. (A) Schematic illustration of the CRISPR-Cas9 ribonucleoprotein (RNP) complex and injection into a one-cell stage zebrafish embryo. (B) Candidate crRNA location and sequence. Double-strand break occurs 2 bases upstream from the stop codon for the selected crRNA. (C) Heteroduplex mobility assay (HMA) to evaluate the cleavage efficiency of crRNA. A 1.5 or 3 fmol RNP complex was microinjected, and genomic DNA was extracted from three pools of five embryos each at 1 dpf. Primers used for HMA are listed in Supplementary Table 1. (D) Percentage of indel mutations by Inference of CRISPR Edits (ICE) analysis.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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