FIGURE

Fig. 1

ID

ZDB-FIG-210301-148

Publication

Zimmer et al., 2020 - Use of a carbonic anhydrase ca17a knockout to investigate mechanisms of ion uptake in zebrafish (Danio rerio)

Other Figures

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Fig. 1

Genotyping and confirmation of ca17a?/? deletion in larval zebrafish (Danio rerio). A: nucleotide alignment of the sequenced ca17a+/+ and ca17a?/? alleles showing the CRISPR sgRNA target sequence in the shaded box and the positions of the 7 nucleotide (nt) deletion and C to T substitution mutations in the red box. B: multiplex PCR products from fin clips of F2 larval offspring of F1 ca17a+/? crosses. The general amplicon (477 base pairs; bp) band appears for all genotypes, the 147-bp band is the ca17a+/+-specific amplicon, and the 352-bp band is the ca17a?/?-specific amplicon. C: representative Western blot of Ca17a and ?-actin protein expression in ca17a+/+, ca17a+/?, and ca17a?/? whole larvae homogenates at 9 days postfertilization (dpf). D: immunofluorescent staining of Ca17a in the yolk sac of ca17a+/+, ca17a+/?, and ca17a?/? 4 dpf larval zebrafish. Scale bar = 50 ?m.

Expression Data

Gene:	ca2
Fish:	WT ca2^ot206/ot206 ca2^ot206/+
Anatomical Terms:	whole organism yolk syncytial layer
Stage Range:	Day 4 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ca2^ot206/ot206 ca2^ot206/+
Observed In:	whole organism yolk syncytial layer
Stage Range:	Day 4 to Days 7-13

Phenotype Detail

Acknowledgments

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