Swimming patterns, sexuality levels, and mating rates of PCOS zebrafish after 3 days (A), 14 days (B), 30 days (C), and 60 days (D) of rearing in different conditions. Left panels showed the static positions of the fish at 15-s intervals during the 30-min recording period. Bold dashed lines indicate the net. In all cases, the PCOS fish that had been previously kept alone displayed preferences to swim in areas close to the net. In contrast, the PCOS fish that had been previously kept with males swam randomly in all areas. Middle panels showed relative sexuality scores of PCOS fish that had been previously kept alone (black bars) or intermixed with males (grey bars). The scores of PCOS zebrafish kept alone were normalized to 1, and the scores of PCOS zebrafish that were kept with males were compared to the base value of 1. Note the decrease in sexuality levels in PCOS fish reared intermixed with males. Right panels showed the mating rate of PCOS zebrafish after they were kept alone (black bars) or intermixed with males (grey bars). After 3 or 14 days of rearing under different conditions, no statistical differences were detected in the mating rates. After 30 or 60 days of different rearing, the mating rate was increased in PCOS fish that had been previously reared alone as compared to PCOS fish that had been previously reared intermixed with males. Data represents the means ± SE (n = 8 in each group). *p < 0.05; **p < 0.01; ns not significant.
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