Fig. 4

Fig. 4. Notch signaling is involved in the proliferation of olig2⁺ RG and parenchymal OPCs. All panels show transverse sections of the telencephalon, with the dorsal side at the top. (A~D?) Labeling of the telencephalon of Tg(olig2:EGFP) adult zebrafish with anti-S100? and -PCNA antibodies. (A, B) Treatment of wildtypes with DMSO (A) and LY-411575 to inhibit Notch signaling (B). Boxed areas indicate the LVZ (A?, B?) and MVZ (A?, B?). Arrows indicate PCNA+/S100?+ proliferating RG (B?), and the arrowhead indicates PCNA⁺/S100?⁺/olig2:EGFP⁺ proliferating RGs (B?). (C, D) Treatment of the injured telencephalon 4 dpl with DMSO (C) and LY-411575 to inhibit Notch signaling (D). Arrows indicate PCNA⁺/S100?⁺ proliferating RGs (C?, D?), and arrowheads indicate PCNA⁺/S100?⁺/olig2:EGFP⁺ proliferating RGs (C?, D?). (E~G) Quantification of proliferating RGs (S100?⁺/EGFP⁺/PCNA⁺) in the medial ventricular zone (E), proliferating parenchymal OPCs (S100?^-/EGFP⁺/PCNA⁺) (F), and proliferating olig2⁺ RGs (S100?⁺/EGFP⁺/PCNA⁺) in the medial ventricular zone (G) (n=9 sections from two zebrafish, Mann?Whitney U test, *p<0.05, **p<0.01, ***p<0.001 and ****p<0.0001). Scale bars: A~D, 100 ?m; A?~D?, 25 ?m. DMSO, dimethyl sulfoxide; EGFP, enhanced green fluorescent protein; LVZ, lateral ventricular zone; MVZ, medial ventricular zone; OPCs, oligodendrocyte progenitor cells; PCNA, proliferating cell nuclear antigen; RG, radial glia.