Fig. 2

Morphogenetic defects in cxcr4b^t26035 and cxcl12a^t30516 mutant embryos resemble those of neurog1^hi1059. (A) Tracks showing migration of anterior EON from control (black), cxcr4b^t26035 (blue) and cxcl12a^t30516 (green) embryos. Twelve anterior tracks are represented (two cells each from the left and right of three embryos). The origin of the tracks has been arbitrarily set to the intersection of the x/y-axes and the early (coloured) and late (grey) phases of migration have been highlighted. (B) Mean tracks showing migration of anterior EON of control (black), neurog1^hi1059 (magenta), cxcr4b^t26035 (blue) and cxcl12a^t30516 (green) mutant embryos. (C) Pairwise PCA scatterplots of morphogenetic parameters extracted from the datasets corresponding to the tracks in B. The major difference between control, neurog1^hi1059, cxcr4b^t26035 and cxcl12a^t30516 mutant embryos (PC1) corresponds to the antero-posterior axis. (D) Clustering analysis of morphogenetic parameters extracted from the datasets presented in B and analysed in C. One cluster, k1, contains almost exclusively control cells (black), whereas cells from neurog1^hi1059 (magenta), cxcr4b^t26035 (blue) and cxcl12a^t30516 (green) mutant embryos clustered together in k2, k3 and k4.