FIGURE

Fig. 3

ID

ZDB-FIG-210208-8

Publication

Reinoß et al., 2020 - Hypothalamic Pomc Neurons Innervate the Spinal Cord and Modulate the Excitability of Premotor Circuits

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Fig. 3

Figure 3. Manipulation of the Mc4r System Affects Locomotor Activity in Zebrafish and Mice (A) Behavior paradigm for video recordings of fasted or sated larvae at 8–9 mm SL. (B) Representative trajectories of 5 min high-magnification video recordings of a fasted and sated larva in the presence of paramecia (prey), with individual paramecia uptake events indicated by purple circles. (C) Plot shows velocity over time and the threshold (dashed line, 6 mm/s) to detect a swim bout. (D) Plots depicting increased locomotion of fasted compared to sated larvae during foraging and feeding (Error bars show 95% confidence intervals; ∗p < 0.05, ∗∗p < 0.01; N = 12). (E and F) Distance traveled in presence of food in fasted (E) and sated (F) larvae (Error bars show 95% confidence intervals; columns with the same superscript letter (a, b, c) are not significantly different (p > 0.05); N = 12). (G) Experimental schematic in mice (left) and expression of Cre-dependent AAV-FLEX-hGlyR-mCherry in ARC^Pomc neurons (right) as determined by immunolabeling. Scale bar, 100 μm. (H) Effects of hGlyR/Ivermectin-induced inhibition of mouse ARC^Pomc neurons on locomotor activity in presence of food (Error bars show 95% confidence intervals;∗p < 0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	fasting
Observed In:	larval foraging behavior
Stage:	Days 21-29

Phenotype Detail

Acknowledgments

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