Fig. 3

(A to F) Adult zebrafish movement was recorded with a camera system for 6 hours. Time spent swimming at low (<30 mm/s) (A), medium (30 to 60 mm/s) (C), and high (E) speeds (60 mm/s or above) and count of times low (B), medium (D), and high (F) swimming speeds were plotted. Each of the 18 fish from either genotype was recorded twice (n = 36) and shown as average ± SEM. P values were calculated by two-tailed Student’s t test with Holm adjustment for multiple comparisons. (G to J) Four-dpf (G and H) and 5-dpf (I and J) embryos from a single female tdp1^−/− and male tdp1^−/+ incross were subjected to 3 cycles of 5-min darkness and 5-min light using the photomotor response assay. (G and I) Total distance traveled in each cycle was plotted in each data point as average ± SEM. P value, two-tailed Student’s t test. (H and J) Total distance traveled in all light or dark cycles was measured. Lines indicate average ± SEM. (I) Total distance traveled each cycle was plotted as average ± SEM. P values were calculated using two-tailed Student’s t test. DMSO, dimethyl sulfoxide. *P < 0.05; **P < 0.01.