Fig. 7

Cells from haploid and tetraploid embryos migrate suboptimally during gastrulation. Cell migration quantification from shield to bud stages of gastrulation in mosaic (A–D) and chimeric embryos (E–Q). For comparisons across experimental conditions and individual timelapses, the quantifications shown are for a normalized duration of 60 ​min. Graphs show the distribution of total distance travelled (A), displacement (B), tortuosity (C) and speed (D) of 90 ​cells from 3 diploid (D), 4 haploid (H) and 4 tetraploid (T) embryos. Diploid red cells co-transplanted with diploid green cells tend to mix during migration (E–G). Diploid (red) cells co-transplanted with haploid or tetraploid (green) cells into diploid hosts at sphere stage (4hpf, H, K) and imaged through gastrulation (I, J, L, M), in which the haploid and tetraploid cells are always farther away from the dorsal shield (D). Graphs show the distribution of total distance travelled (N), displacement (O) tortuosity (P) and speed (Q) of 80 diploid (D, red), haploid (H, green) and tetraploid (T, green) from 5 host embryos of each co-transplant. Asterisks denote statistical significance based on Mann Whitney (p ​< ​0.0001, 0.001 and ​< ​0.05) and dotted horizontal line within box plots indicate mean value. Dots represent individual cells and colours of the dots represent individual embryos quantified from independent experiments.