FIGURE

Fig. 3

ID

ZDB-FIG-201113-24

Publication

Cook et al., 2020 - Visualisation of cholesterol and ganglioside GM1 in zebrafish models of Niemann-Pick type C disease and Smith-Lemli-Opitz syndrome using light sheet microscopy

Other Figures

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Fig. 3

Cholesterol is reduced in SLOS model zebrafish and increased in NPC model zebrafish. Representative images of filipin staining of 96 hpf zebrafish to observe cholesterol distribution; a Filipin staining of SLOS zebrafish models induced using either the Dhcr7 inhibitor AY9944 (75 µM) or dhcr7 morpholino (dhcr7 MO) injected at the 1–2 cell stage. Images of the zebrafish head and upper body from a lateral view (left) or dorsal view (right); b Filipin staining of NPC zebrafish models induced using either npc1 morpholino (npc1 MO) injected at the 1–2 cell stage or the Npc1 inhibitor, U18666A (2 µg/ml). Images of the zebrafish head from a dorsal viewpoint. Brain areas are indicated by coloured bars above images. Scale bars = 280 µm. N = 3, with a minimum of ten fish per N

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL AB/TL + MO1-dhcr7 AB/TL + MO1-npc1
Conditions:	chemical treatment: N-[(2-chlorophenyl)methyl]-1-[4-[[(2-chlorophenyl)methylamino]methyl]cyclohexyl]methanamine chemical treatment: amphiphile
Knockdown Reagents:	MO1-dhcr7 MO1-npc1
Observed In:	cerebellum cholesterol forebrain cholesterol hindbrain cholesterol midbrain cholesterol
Stage:	Day 4

Phenotype Detail

Acknowledgments

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