FIGURE

Fig. 5

ID

ZDB-FIG-201113-10

Publication

Bosze et al., 2020 - Pcdh18a regulates endocytosis of E-cadherin during axial mesoderm development in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

Pcdh18a affects E-cadherin dependent cell migration. a Wound-healing assay in HeLa cells. Cells were transfected with the indicated constructs and their migratory behaviour was monitored for 10 h after removing the insert. The dotted line shows limits of the confluent cell layer. b Quantification of the migration speed of HeLa cells. c Wound-healing assay in L cells. Cells were transfected with indicated constructs. After 24 h, L cells were treated with DMSO (1%) or Dyngo4a endocytosis inhibitor (1 µM in DMSO 1%). The migratory behaviour of cells was monitored in a time lapse for 34 h. d Quantification of the migration speed of L cells after blocking endocytosis. All wound-healing assays were conducted in independent triplicate, distances of the gap were measured at ten fixed positions. Mean values, SEM and significance are indicated (*p value < 0.05, ***p value < 0.005; unpaired Student’s t test). Scale bar: 200 µm

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Histochem. Cell Biol.